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, treated with OSI-027 or rapamycin, and examined by confocal microscopy for autophagosome formation. As indicated in supplemental Figure 3A-B, OSI-027 and rapamycin increased autophagy to the same extent. In further experiments, cells were treated with OSI-027 in the absence and presence of 3-methyladenine or chloroquine, 2 reported autophagy inhibitors.37 As indicated in supplemental Figure 3C,

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