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M Localization of Acetyl-K40 on Microtubulesneedle. Specific antibodies were affinity purified by adsorption to the same peptide coupled to a Sulfolink column (Pierce). The following monoclonal antibodies were purchased: antiacetylated tubulin clone 6-11B-1 ([5] Sigma T7451), anti-a-tubulin clone DM1A (Sigma T6199), and anti-b-tubulin clone E7 (Developmental Studies Hybridoma Bank). Secondary anti

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